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biac:analysis:resting_pipeline [2012/07/09 18:57] – [Python/FSL Resting State Pipeline] adminbiac:analysis:resting_pipeline [2012/07/09 19:02] – [Step 4] admin
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   * the functional run is meaned across time with fslmaths, then bet is applied.  the resulting mask is then applied to the entire run of data   * the functional run is meaned across time with fslmaths, then bet is applied.  the resulting mask is then applied to the entire run of data
   * if you find that bet is doing a poor job finding the edges you can adjust the intensity threshold with **--betfval**   * if you find that bet is doing a poor job finding the edges you can adjust the intensity threshold with **--betfval**
-    * this is equivalent of **-f** when actually running bet.  the default is 0.5, but smaller values will be more conservative in finding the edges of the brain ( ie: larger mask ) +    * this is equivalent of **-f** when actually running bet.  the default is 0.4 (same as feat), but smaller values will be more conservative in finding the edges of the brain ( ie: larger mask ) 
 +  * if provided T1 anatomical is skull stripped. **--anatbetfval** is used to control intensity threshold, the default is 0.5 (same as feat)
 ==== Step 4 ==== ==== Step 4 ====
   * normalize the data using flirt   * normalize the data using flirt
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   * if your subject has already been normalized during standard pre-processing of other runs, please provide the flirt matrix from pre-stats with **--flirtmat** ( most likely example_func2standard.mat)   * if your subject has already been normalized during standard pre-processing of other runs, please provide the flirt matrix from pre-stats with **--flirtmat** ( most likely example_func2standard.mat)
     * this will apply the previously determined flirt matrix to your functional data instead of trying to calculate a new matrix based on the functionals.  the matrix from pre-stats was likely calculated using a high-resolution anatomical, also this will assure that your resting state runs are in the same space as the other runs from your subject.     * this will apply the previously determined flirt matrix to your functional data instead of trying to calculate a new matrix based on the functionals.  the matrix from pre-stats was likely calculated using a high-resolution anatomical, also this will assure that your resting state runs are in the same space as the other runs from your subject.
 +  * if you've provided at T1 anatomical image then this sequence is followed:
 +    * func-2-t1
 +    * t1-2-standard
 +    * flirt matrices are concatenated to create func-2-standard
 +  * if no T1 is provided, then the functional is used for the flirt normalization
  
 ==== Step 5 ====  ==== Step 5 ==== 
biac/analysis/resting_pipeline.txt · Last modified: 2024/06/21 15:44 by 127.0.0.1